Mélisande BLEIN-NICOLAS

Mélisande BLEIN-NICOLAS

 dernières mises à jour 08/10/2024 -   01 69 15 68 06 -  1219
 melisande.blein-nicolas@inrae.fr
 Plateforme d'Analyse Protéomique de Paris Sud-Ouest
 Biologie de l'Adaptation et Systèmes en Évolution
 INRAE, Ingénieure de Recherche
 Proteomics, mass spectrometry, systems biology, omics data integration, plant genetics
 publications - orcid  - HAL

Position and education

  • 2015-present: INRAE research engineer, UMR GQE-Le Moulon, Gif-sur-Yvette, France
  • 2010-2015: Post-doctoral fellow, INRAE, UMR GQE-Le Moulon, Gif-sur-Yvette, France
    • Analysis of heterosis for protein abundance under normal and drought conditions in sunflower
    • Genome wide association study for protein expression under normal and water deficit conditions in maize leaves
    • Analysis of heterosis for protein abundance in inter- and intra-specific yeast hybrids grown under grape must fermentation
  • 2008-2010: Postdoctoral fellow, CIRAD, UMR Biologie et Génétique des Interactions Plante-Parasite, Montpellier, France
    • Role of the CIGR1 gene in the resistance of rice to blast
  • 2004-2007: PhD student (MENRT fellowship), IGEPP   (formely UMR Amélioration des Plantes et Biotechnologies Végétales), Rennes, France
    • Role of the Pch1 gene in the resistance of wheat to eyespot
  • 2004: Master of Science and Engineer degrees in Plants Genetics and Breeding, Agrocampus Ouest (formerly École Nationale Supérieure Agronomique de Rennes).

Responsabilities

  • 2022-present: Scientist-in-charge of the PAPPSO   core facility
  • 2017-2023: member of the executive board of the French Proteomics Society ( FPS   )

Activities of research and development

I am interested in understanding how living organisms respond and adapt to environmental changes. My research focuses on identifying genes and molecular mechanisms responsible for variations in adaptive traits, such as plant resistance to pathogenic fungi, the ability of yeasts to complete grape juice fermentation, and drought tolerance in plants. During my PhD and first postdoctoral fellowship, I addressed these issues using functional genomics approaches. Since joining UMR GQE-Le Moulon, I have transitioned to systems approaches that integrate multi-omics data to elucidate the genotype-phenotype relationship. My current research is centered on the genetic control and plasticity of molecular networks involved in drought response in maize. As the scientist-in-charge of the PAPPSO facility, I am also developing high-throughput plant proteomics approaches to enhance the application of proteomics in research programs related to plant breeding and agroecology, which require the analysis of large cohorts of samples.

Teaching and training

  • Introduction to mass-spectrometry based proteomics (Licence 1 and Master 1 and 2 at Université Paris Saclay)
  • Proteomics Research School: “From Sample Preparation to Results Interpretation—Strategies and Practical Aspects” organized in the framework of the MassProt’INRAE   network

Publications

  • Langella O., Renne T., Balliau T., Davanture M., Brehmer S., Zivy M., Blein-Nicolas M. , Rusconi F.. (2024) Full native timsTOF PASEF-enabled quantitative proteomics with the i2MassChroQ software package. Journal of Proteome Research,
  • Tarkowski Łukasz P., Clochard T., Blein-Nicolas M. , Zivy M., Baillau T., Abadie C., Morère-Le Paven MC., Limami AM., Tcherkez G., Montrichard F.. (2024) The nitrate transporter-sensor MtNPF6.8 regulates the branched chain amino acid/pantothenate metabolic pathway in barrel medic (Medicago truncatula Gaertn.) root tip. Plant Physiology and Biochemistry, (206) 108213
  • Chasseriaud L., Albertin W., Blein-Nicolas M. , Balliau T., Zivy M., Coulon J., Bely M.. (2023) Physical Contact between Torulaspora delbrueckii and Saccharomyces cerevisiae Alters Cell Growth and Molecular Interactions in Grape Must. Beverages, 3 (9) 81
  • Duruflé H., Balliau T., Blanchet N., Chaubet A., Duhnen A., Pouilly N., Blein-Nicolas M. , Mangin B., Maury P., Langlade NB., Zivy M.. (2023) Sunflower Hybrids and Inbred Lines Adopt Different Physiological Strategies and Proteome Responses to Cope with Water Deficit. Biomolecules, 7 (13) 1110
  • Jamet E., Esquerré-Tugayé MT., Gallardo-Guerrero K., Rolland N., Zivy M., Blein-Nicolas M. , Vincent D., Gontero B., Rajjou L.. (2023) Obituary: Dominique Job (1947-2022). Front. Plant Sci., (14) 1188766
  • Prunier G., Cherkaoui M., Lysiak A., Langella O., Blein-Nicolas M. , Lollier V., Benoist E., Jean G., Fertin G., Rogniaux H., Tessier D.. (2023) Fast alignment of mass spectra in large proteomics datasets, capturing dissimilarities arising from multiple complex modifications of peptides. BMC Bioinformatics, 1 (24) 421
  • Baudouin E., Puyaubert J., Meimoun P., Blein-Nicolas M. , Davanture M., Zivy M., Bailly C.. (2022) Dynamics of Protein Phosphorylation during Arabidopsis Seed Germination. International Journal of Molecular Sciences, 13 (23) 7059
  • Millán-Oropeza A., Blein-Nicolas M. , Monnet V., Zivy M., Henry C.. (2022) Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes, 1 (10) 2
  • Plancade S., Berland M., Blein-Nicolas M. , Langella O., Bassignani A., Juste C.. (2022) A combined test for feature selection on sparse metaproteomics data—an alternative to missing value imputation. PeerJ, (10) e13525
  • Bassignani A., Plancade S., Berland M., Blein-Nicolas M. , Guillot A., Chevret D., Moritz C., Huet S., Rizkalla S., Clément K., Doré J., Langella O., Juste C.. (2021) Benefits of Iterative Searches of Large Databases to Interpret Large Human Gut Metaproteomic Data Sets. J. Proteome Res.,
  • Urrutia M., Blein-Nicolas M. , Prigent S., Bernillon S., Deborde C., Balliau T., Maucourt M., Jacob D., Ballias P., Bénard C., Sellier H., Gibon Y., Giauffret C., Zivy M., Moing A.. (2021) Maize metabolome and proteome responses to controlled cold stress partly mimic early-sowing effects in the field and differ from those of Arabidopsis. Plant Cell Environ,
  • Blein-Nicolas M. , Negro SS., Balliau T., Welcker C., Cabrera-Bosquet L., Nicolas SD., Charcosset A., Zivy M.. (2020) A systems genetics approach reveals environment-dependent associations between SNPs, protein coexpression, and drought-related traits in maize. Genome Res., 11 (30) 1593-1604
  • Belouah I., Nazaret C., Pétriacq P., Prigent S., Bénard C., Mengin V., Blein-Nicolas M. , Denton AK., Balliau T., Augé S., Bouchez O., Mazat JP., Stitt M., Usadel B., Zivy M., Beauvoit B., Gibon Y., Colombié S.. (2019) Modeling Protein Destiny in Developing Fruit. Plant Physiology, 3 (180) 1709-1724
  • Belouah I., Blein-Nicolas M. , Balliau T., Gibon Y., Zivy M., Colombié S.. (2019) Peptide filtering differently affects the performances of XIC-based quantification methods. J Proteomics, (193) 131-141
  • Henry C., Haller L., Blein-Nicolas M. , Zivy M., Canette A., Verbrugghe M., Mézange C., Boulay M., Gardan R., Samson S., Martin V., André-Leroux G., Monnet V.. (2019) Identification of Hanks-Type Kinase PknB-Specific Targets in the Streptococcus thermophilus Phosphoproteome. Front. Microbiol., (10) 1329
  • Langella O., Valot B., Balliau T., Blein-Nicolas M. , Bonhomme L., Zivy M.. (2017) X!TandemPipeline: A Tool to Manage Sequence Redundancy for Protein Inference and Phosphosite Identification. J. Proteome Res., 2 (16) 494-503
  • Millan-Oropeza A., Henry C., Blein-Nicolas M. , Aubert-Frambourg A., Moussa F., Bleton J., Virolle MJ.. (2017) Quantitative Proteomics Analysis Confirmed Oxidative Metabolism Predominates in Streptomyces coelicolor versus Glycolytic Metabolism in Streptomyces lividans. J. Proteome Res., 7 (16) 2597-2613
  • Blein-Nicolas M. , Zivy M.. (2016) Thousand and one ways to quantify and compare protein abundances in label-free bottom-up proteomics. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 8 (1864) 883-895